The youPCR® Plant Screening Platform allows hemp and cannabis cultivators to screen plants for genetic traits and pathogens, while they are still in the seedling tray. Early screening can be used to prevent costly infestations and save valuable time and resources that would otherwise be spent on plants with undesirable traits, such as males.
Virtually eliminate time and resources transplanting, watering, and monitoring male plants that will never produce flowers. With the youPCR® Gender Detection test, you can identify and eliminate male plants just one week after germination, giving you more time to care for your productive females and optimize your operation.
Get early feedback on your efforts to breed strains with specific cannabinoid ratios. The youPCR® CBDA Marker and youPCR® THCA Marker tests can determine if a plant has the genes needed to produce CBDA and/or THCA, just one week after germination. This will help you better select plants with desirable traits that will be used in subsequent crosses.
The youPCR® Platform can identify plants infected with powdery mildew, russet mites, or botrytis before they show visual symptoms. Use youPCR® to screen your incoming clones to make sure you are not introducing infected plants to your grow, and continue to screen plants throughout their life so you can keep pathogens in check.
Eliminate males before they use up valuable lighting and HVAC resources that could be better spent on productive females.
Stop wasting valuable water and nutrients on plants that do not have the gender or dominant cannabinoid you are looking for.
Early removal of plants that do not have the gender or dominant cannabinoid you are looking frees up more space that you can fill with the plants you want.
Spend less time inspecting plants for visual signs of contamination or gender, and spend more controlling the environment and taking good notes.
1. Collect 4mm punches from each plant leaf and place each in one well of the pre-aliquoted Solution A strip tube.
2. Heat the tubes, with either a thermal cycler or miniPCR machine, for 50 minutes.
All current and future youPCR® tests can be run on either a 16-well miniPCR thermal cycler or a standard 96-well thermal cycler. Customers should consider volume when choosing which solution will work in their instance. The miniPCR platform is best for small grows or home grows, while larger commercial grows will typically be better suited for a 96-well thermocycler. Contact a Medicinal Genomics representative who can help determine the right solution for your situation.
Contact Medicinal Genomics to acquire a discount coupon code that can be used when purchasing from the miniPCR website.
Any programmable 96-well thermocycler with a heated lid will work. qPCR detection is not required.
No. Although we have done some initial research into using youPCR to test seeds, we have not done enough to confidently say it works as well as testing leaf. We recommend sampling a seedling’s first set of true leaves when using you PCR.
YouPCR assays provide a positive/negative result. When using youPCR to screen for CBDA or THCA synthase, a positive result means there is an active CBDA or THCA synthase allele. Unfortunately, that result can’t be used to predict what percentage the plant will ultimately produce. However, if you combine the CBDA and THCA tests you can determine the plant type.
No. Cannabis plants typically hermie as a response to stress. Although there may be genetic markers that determine if a plant is more likely to hermie, we have not found them, and we do not target them with our gender tests. The youPCR gender assay specifically looks for a Y chromosome.
The youPCR Starter Kits do not include PCR Thermal Cyclers. The youPCR Starter Kits also do not include chemical reagents: Solution A, Solution B, Solution C, and Positive Control. Those must be purchased separately on the Medicinal Genomics web store.
In order to successfully test with youPCR, you should have experience with the following laboratory techniques/best practices:
All of the chemicals and reagents we sell in our web store are manufactured in facilities that are certified to ISO 9001 and/or ISO 13485 standards.
Cultivators who don’t have enough volume, lab experience, or start-up cash to test seedlings in-house can outsource to one of our partner labs. They have great turnaround time and aren’t going to use your genetic data to fuel a breeding operation.
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