Eliminate free DNA from dead cells so it does not inflate quantitative results from PathoSEEK® qPCR Detection Assays. Grim Reefer® provides a quick, accurate, and affordable solution to a problem that has haunted labs using DNA-based methods.
Grim Reefer® reagents are safer to handle than ethidium monoazide (EMA) or propidium monoazide (PMA) treatments.
The Grim Reefer® method adds just 10 minutes to the SenSATIVAx® DNA Purification Process. Much faster than re-running after incubation.
Grim Reefer® performs better than other enzymatic solutions, ethidium monoazide (EMA), or propidium monoazide (PMA) treatments.
Add the Grim Reefer® buffer and enzyme to the sample and
incubate at 37C for 10 minutes to degrade any dead of free DNA. After incubation, add MGC lysis buffer to the sample and mix well. The lysis buffer inactivates the Grim Reefer® Enzyme while also lysing the viable cells. After the Lysis step, add the Grim Reefer® Positive Control, which contains a known amount of free DNA, to ensure the enzyme is deactivated. If the Grim Reefer® Positive Control appears in the qPCR results, you know the Grim Reefer® Enzyme was properly deactivated.
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Add Grim Reefer® Enzyme and Buffer to homogenized sample and incubate for 10 minutes.
Grim Reefer® Enzyme eats any free DNA present in the sample
Add MGC Lysis Buffer
Lysis Buffer deactivates Grim Reefer® Enzyme and lyses viable cells to expose DNA
Add Grim Reefer® Positive Control
Positive Control is Free DNA used to show the Grim Reefer® Enzyme has been deactivated
A new method for preventing dead DNA from inflating qPCR results
It is not recommended that Free DNA Removal protocols be used with assays that require an enrichment such as Aspergillus, Salmonella and STEC. There is a simpler more cost effective alternative.
Remediation techniques, such as radiation, may damage plant cell walls and create more exposed cannabis DNA, which the Grim Reefer enzyme will chew up.
Our genetics-based cannabis testing and breeding technologies can help growers, dispensaries and labs grow cleaner, faster and healthier in every way. Because you’re not just delivering a product. You’re building a business. And a reputation.
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