Specifically designed for use on cannabis and hemp products, including flower, extracts, and a variety of infused products. The PathoSEEK® platform uses qPCR technology, an established safety testing method in the food industry and medicine that has been used in several peer-reviewed studies.
Targeting organisms on a genetic level is a better strategy than culture-based methods, which often grow off-target organisms, leading to false positives and more pesticide usage.
Stop waiting for plating. PathoSEEK® delivers same-day results for total count tests and next-day results for presence-absence tests.
DNA extraction and qPCR setup can be automated on liquid handling robots to increase throughput by 500%, while requiring only 2 hours of hands-on time per day.
Cannabis DNA Internal Control
All PathoSEEK® qPCR assay internal controls target a sequence in the cannabis genome, which helps to ensure plant cells have been lysed to release pathogens growing endophytically, DNA survived extraction intact, and there is no contamination in your Assay Master Mix.
Primers and Probes Designed for Cannabis
All PathoSEEK® primers and probes were tested in-silico against the cannabis genome to ensure no cross-reactivity. Other platforms that use primers and probes borrowed from food testing may inadvertently hit the cannabis genome.
Universal Cycle Time
All PathoSEEK® assays use the same cycle program, so you can run several different assays simultaneously.
Turnkey Automation
DNA purification and qPCR setup can be fully automated with turnkey solutions from Agilent Technologies, Hamilton Robotics, and MGI/BGI.
K McKernan, S McLaughlin, N Houde, LT Kane, Y Helbert, L Zhang, S Hom
Zenodo
Biocontrol agents and their influence on the cannabis testing space
K McKernan, K Marsh, S Cottrell, S Hom
OSF Preprints
K McKernan, Y Helbert, LT Kane, L Zhang, N Houde, A Bennett, J Silva, …
F1000Research 11 (578), 578
K McKernan, Y Helbert, L Kane, N Houde, L Zhang, S McLaughlin
F1000Research 10
KJ McKernan, Y Helbert, LT Kane, H Ebling, L Zhang, B Liu, Z Eaton, …
BioRxiv
K McKernan, J Spangler, Y Helbert, RC Lynch, A Devitt-Lee, L Zhang, …
F1000Research 5
Microbiological examination of nonsterile Cannabis products
K McKernan, Y Helbert, H Ebling, A Cox, LT Kane, L Zhang
OSF Preprints
K McKernan, J Spangler, L Zhang, V Tadigotla, Y Helbert, T Foss, D Smith
F1000Research 4
PathoSEEK® 5-Color Aspergillus Multiplex Assay with SenSATIVAx® Extraction
PathoSEEK® 2-Color Aspergillus Multiplex Assay with SenSATIVAx® DNA Purification
PathoSEEK® Salmonella & STEC Multiplex Assay with SenSATIVAx® Extraction
PathoSEEK® Total Aerobic Count Detection Assay with SenSATIVAx® Extraction
PathoSEEK® Total Yeast and Mold Count Assay with SenSATIVAx® & TLP Extraction
PathoSEEK® Bile Tolerant Gram Negative Detection Assay v2 with SenSATIVAx® Extraction
PathoSEEK® Total Coliform & Enterobacteriaceae Assay with SenSATIVAx® Extraction
All Medicinal Genomics products are manufactured in ISO certified facilities. MGC develops and tests all of its reagents in house, but outsources the manufacture of our commercial products to ISO certified partners.
Real-time or quantitative PCR (qPCR) provides more consistent, reproducible results and is more sensitive than end-point PCR
PathoSEEK® qPCR assays WILL work on Quant Studio instruments, but certain settings and calibrations are required, and Medicinal Genomics will be limited with what support and troubleshooting it can provide.
Yes. PathoSEEK’s Aspergillus 5-Color Multiplex and Salmonella/STEC E.coli Multiplex assays have been certified by the AOAC for use on cannabis flower, concentrates, and infused edibles
There are a number of reasons why qPCR results and plating results may disagree when the methods are compared in a real-life scenario:
Our genetics-based cannabis testing and breeding technologies can help growers, dispensaries and labs grow cleaner, faster and healthier in every way. Because you’re not just delivering a product. You’re building a business. And a reputation.
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