Using RT-qPCR and Tissue Culture to Control Hop Latent Viroid in Cannabis Cultivation

Hop Latent Viroid (HLVd) was first identified as associated with “dudding” disease in Cannabis in 2019. It has since rapidly spread through Cannabis cultivation facilities worldwide, causing significant yield and potency losses. HLVd can now be found in most US cannabis cultivation operations. The annual revenue lost in the US is estimated to be up to $4 billion. Strategies to control HLVd include prevention, testing, and culling infected plants.

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